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cap biotin avanti lipids  (Croda International Plc)

 
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    Structured Review

    Croda International Plc cap biotin avanti lipids
    Cap Biotin Avanti Lipids, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 98/100, based on 658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cap+biotin+avanti+lipids/18%3A1+DGS-NTA/pm41429941-142-155-156
    Average 98 stars, based on 658 article reviews
    cap biotin avanti lipids - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Recombinant:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.

    Sequencing:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.

    Cell Stimulation:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.

    Isolation:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.

    Software:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.

    Cell Isolation:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.

    Activation Assay:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.

    Expressing:

    Article Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.
    Article Snippet: N/A Recombinant DNA LifeAct-mCitrine Addgene Catalog# 54733 LifeAct-EGFP Addgene Catalog# 58470 Antibodies Anti-Mouse CD3ε (Clone 145- 2C11) Biolegend Catalog# 100302 Anti-Human CD3 (Clone OKT3) Biolegend Catalog# 317302 Oligonucleotides and other sequence-based reagents N/A Chemicals, enzymes and other reagents RPMI-1640 GIBCO Catalog # 11875093 Fetal Bovine Serum GIBCO Catalog # A5256701 L-glutamine Thermo Fisher Catalog #A2916801 Sodium Pyruvate Thermo Fisher Catalog # 11360070 Penicillin/Streptomycin Thermo Fisher Catalog # 15140122 PMA/Ionomycin eBioscienceTM Cell Stimulation Cocktail 500X Catalog # 00- 4970-93 TheraPEAK® X-VIVO® 10 Serum-free Hematopoietic Cell Medium Lonza Catalog #: BEBP02-055Q Human IL-2 Recombinant Protein PeproTech Catalog # 200- 02-50UG HEPES Gbioscience Catalog #:RC060 6X His-ICAM1 Thermo Fisher Catalog #10346H03H50 NaCl Merck(Emparta) CAS #7647-14- 5 KCl SDFCL CAS #7447-40- 7 Glucose Sigma-Aldrich CAS #50-99-7 CaCl2 SDFCL CAS #10043- 52-4 MgCl2 Sigma-Aldrich CAS #7786-30- 3 NiCl2 Qualigens CAS #7791-20- 0 Streptavidin Thermo Fisher Catalog # 434301 DOPC Avanti Lipids (SigmaAldrich) Cat# 850375 NTA-DGS Avanti Lipids (SigmaAldrich) Cat# 790404 Cap-Biotin Avanti Lipids (SigmaAldrich) Cat# 870273 Reagent/Resource Reference or Source Identifier or Catalog Number CD8 T-cell isolation kit Stem Cell Technologies Catalog # 19853 CK666 Merck CAS # 442633- 00-3 Jasplakinolide Calbiochem CAS # 102396- 24-7 Latrunculin Calbiochem CAS# 76343- 93-6 Software MATLAB https://www.mathworks. com/products/matlab.html N/A GraphPad Prism8 https://www.graphpad. com/features N/A Adobe Photoshop Adobe N/A Image J https://imagej.net/ij/ N/A Trackpy https://pypi.org/project/ trackpy/ N/A OpenCV https://opencv.org/blog/ edge-detection-usingopencv/ N/A OpenPIV https://openpiv. readthedocs.io/en/latest/ N/A Other 25 mm round #1.5 glass coverslips VWR Catalog# MARI0117650 Cell isolation, culture, activation, and pharmacological treatments Mouse CD8+ T cells were isolated either from wild-type C57BL/6 mice, or from wild-type C57BL/6 and WASP-/- C57BL/6 mice expressing LifeAct-GFP, using the CD8+ T Cell Isolation Kit (Stem Cell Technologies) following the manufacturer’s protocol.



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